Open AccessComparison of growth and cell proliferation kinetics during mouse molar odontogenesis in vivo and in vitro
Author(s) -
Ahmad N.,
Ruch J. V.
Publication year - 1987
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1987.tb01314.x
Subject(s) - ameloblast , odontoblast , in vivo , molar , in vitro , mitosis , microbiology and biotechnology , andrology , biology , cell growth , chemistry , enamel paint , anatomy , pathology , biochemistry , dentistry , dentin , medicine , genetics , paleontology
. The growth of embryonic first lower mouse molars in vitro was slow and reduced in comparison with in vivo development: the volume of teeth removed on day 15 and 16 of gestation and cultured for 6 days did not exceed the volume reached at day 18 in vivo . The volume of teeth removed on day 17 and 18 and cultured for 6 days either remained constant or decreased. The appearance of post‐mitotic odontoblasts and ameloblasts was delayed in vitro . This behaviour might be correlated with a lengthening of the cell cycle ( T c ). In vitro , the average durations of T c (established by the percentage labelled mitoses technique) were 17.4–20.2 hr and 19.1–19.4 hr for pre‐odontoblasts and pre‐ameloblasts respectively. In vivo , the corresponding T c values were 13.9 hr and 13.5 hr. The coordination of mitotic activities of pre‐odontoblasts and pre‐ameloblasts existing in vivo was maintained in vitro , and therefore seemed to require intra‐dental control mechanisms. Non‐specific extra‐dental serum factors may affect the duration of T c .