Open AccessThe production of factors regulating the proliferation of haemopoietic spleen colony‐forming cells by bone marrow macrophages
Author(s) -
Wright E. G.,
Lorimore S. A.
Publication year - 1987
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1987.tb01098.x
Subject(s) - bone marrow , spleen , biology , microbiology and biotechnology , progenitor cell , stem cell , immunology , cell growth , radioresistance , colony stimulating factor , cell culture , haematopoiesis , biochemistry , genetics
. Media conditioned by normal murine bone marrow cells contain an inhibitor of haemopoietic spleen colony‐forming cell proliferation that is concentrated in a nominal 50‐100K fraction. Media conditioned by regenerating marrow cells contain a proliferation‐stimulatory activity that is concentrated in a nominal 30‐50K fraction. Cell separation experiments demonstrated that the activities are produced by adherent, phagocytic, radioresistant, Thy 1.2 ‐ Fc + , F4/80 + cells. Cultured macrophages, obtained from long‐term marrow cultures or derived from progenitor cells in methyl cellulose cultures are also capable of producing inhibitory and stimulatory activities. The results are consistent with macrophages being an important source of stem cell proliferation regulators in the bone marrow.