Open AccessImplications of disaggregation procedures on biological representation of human solid tumours
Author(s) -
Costa A.,
Silvestrini R.,
Bino G. Del,
Motta R.
Publication year - 1987
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1987.tb01096.x
Subject(s) - cell , human breast , ovarian carcinoma , biology , matrix (chemical analysis) , viability assay , carcinoma , melanoma , breast carcinoma , pathology , chemistry , cancer research , biochemistry , cancer cell , cancer , ovarian cancer , chromatography , medicine , breast cancer , genetics
Abstract. This study was designed to define some biological aspects of cell suspensions, obtained by mechanical or enzymatic disaggregations, and to verify whether single cell suspensions are representative of original solid tumours. The study was performed on a series of 25 human solid tumours including breast carcinoma, ovarian carcinoma and malignant melanoma. A higher cell viability and a loss of aneuploid subpopulations, or a lower fraction of aneuploid cells, were observed in enzymatically‐released samples than in samples obtained by the mechanical procedure. Moreover, the proliferative activity, which was generally similar for the cell suspensions obtained by the two disaggregation procedures, was always markedly lower in the cell suspensions than in solid samples from the same tumour. In conclusion, the results from this study indicate that many changes, such as selective release of cell populations from the tumour matrix, damage and destruction of aneuploid and proliferating cells can be induced to various extents by different disaggregation procedures.