Open AccessRepopulation of spleens of irradiated mice after injection of spleen cell subpopulations
Author(s) -
Girard R.,
Metezeau P.,
Chaby R.
Publication year - 1987
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1987.tb01084.x
Subject(s) - splenocyte , spleen , biology , progenitor cell , immunology , stem cell , repopulation , microbiology and biotechnology , myeloid , haematopoiesis
. Haemopoietic stem cells present in the spleen of adult mice were analysed by grafting X‐irradiated animals with polystyrene‐nonadherent (NABS) and polystyrene‐adherent (ABS) B‐enriched splenocytes from syngeneic donors. The progeny of the haemopoietic stem cells present in NABS and ABS subsets were studied with respect to size, surface markers, and response to mitogens and antigens. Ninety‐six per cent of the precursors of the myeloid cell lineage (CFU‐S) were present in the NABS fraction (50‐fold enrichment). The presence in NABS of progenitors of functional T and B lymphocytes was also demonstrated. Twelve days after grafting with NABS, more than 80% of the recipient splenocytes were large and nonadherent granulocyte‐like cells. These cells had surface similarities with NABS from normal mice, since both populations reacted with peanut agglutinin and with a rabbit anti‐NABS (RAN) serum.