Open AccessEffects of Puromycin, Cycloheximide and Noradrenaline On Cell Migration Within the Crypts and On the Villi of the Small Intestine
Author(s) -
Kaur P.,
Potten C. S.
Publication year - 1986
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1986.tb00762.x
Subject(s) - cycloheximide , crypt , puromycin , lamina propria , epithelium , microbiology and biotechnology , biology , cell , cell migration , thymidine , protein biosynthesis , anatomy , biochemistry , endocrinology , in vitro , genetics
. The normal process of cell migration, occurring as part of the replacement scheme within the small intestinal epithelium, was investigated extensively. the effects of puromycin, cycloheximide and noradrenaline on the movement of tritiated thymidine ([ 3 H]TdR) prelabelled crypt or villus cells have been studied. These studies have led to the formulation of a model for the mechanism of cell migration, postulating that the crypts and villi behave as separate units, with regard to cell migration, in addition to their distinct structural and functional properties. It is proposed that crypt cell migration is an active process requiring protein synthesis and protein glycosylation, whilst movement of villus epithelial cells is passive, depending on the continued contraction of smooth muscle cells in the lamina propria.