Open AccessPluripotent Haemopoietic Stem Cell Entry Into Dna Synthesis In Vitro After Stimulation By Ara‐C Treatment In Vivo
Author(s) -
Izumi H.,
Guigon M.,
Frindel E.
Publication year - 1986
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1986.tb00746.x
Subject(s) - induced pluripotent stem cell , in vivo , bone marrow , microbiology and biotechnology , in vitro , stem cell , cell , medullary cavity , biology , dna synthesis , suspension culture , cell culture , chemistry , immunology , anatomy , biochemistry , embryonic stem cell , genetics , gene
. Non‐cycling pluripotent bone marrow stem cells (CFU‐S), taken 3 hrs after injection of 20 mg of Ara‐C, have been shown to enter DNA synthesis at 1 to 3 hr after being cultured in α‐medium. This phenomenon was observed when bone marrow was incubated as a plug, but not when incubated as a cell suspension in the present experimental conditions. These results suggest that a medullary structure is necessary in order to observe this effect and/or that accessory cells are destroyed during the process of single cell preparation.