Open AccessNegative Effect of Iodide On the Survival of Newly Divided Epithelial Cells In Chronically Stimulated Rat Thyroid
Author(s) -
Rogi J. B.,
Penel C.,
Golstein J.,
Galand P.,
Dumont J. E.
Publication year - 1986
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1986.tb00742.x
Subject(s) - propylthiouracil , iodide , iodine , medicine , endocrinology , thyroid , chemistry , hormone , triiodothyronine , organic chemistry
. The aim of this work was to investigate some aspects of the thyroid epithelial cell kinetics during the iodide‐induced involution of a hyperplastic goitre in the rat. Rats were made iodine‐deficient for 6 months, and propylthiouracil (PTU) (0.15%) was added to the diet during the last 2 months. Thereafter, rats were refed with iodide and PTU was removed (day 0). Forty‐eight hours previously, all the rats were injected with tritiated thymidine ([ 3 H]TdR) (1 μCi/g). Some animals were killed 1 hr or 24 hr after [ 3 H]TdR injection (i.e. on day ‐2 and ‐1, day O corresponding to the restoration of a normal iodine diet); the other animals were killed after different delay periods and following L 3 H]TdR injection. Autoradiography of thyroid sections, iodine determination of plasma iodide and protein‐bound iodine (PBI), and RIA of plasma thyroid stimulatory hormone (TSH) were performed. Plasma TSH concentration was very high on day O of iodide refeeding (3000 ± 330 ng/ml) and remained at this level until day 8. Plasma PBI was very low on day O, remained so until day 4 and greatly increased on day 8. Plasma iodide was also very low on day O, but markedly increased on day 1, then did not vary significantly until day 43 of iodine refeeding. Thyroid weight, elevated on day O, decreased relatively quickly until day 30, then more slowly until day 73.