Open AccessEffects of Hydroxyurea On the Mitotic Activity and the G 2 Phase In Hairless Mouse Epidermis
Author(s) -
Iversen Olav Hilmar,
Harket Gunvald,
Rabe Ragnar
Publication year - 1986
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1986.tb00678.x
Subject(s) - colcemid , hairless , mitosis , biology , epidermis (zoology) , microbiology and biotechnology , andrology , anatomy , genetics , medicine
Hairless mice were given 5 mg hydroxyurea (HU) intraperitoneally (i.p.) followed by 0.15 mg Colcemid® at various times after HU. the animals were killed at 2 and 4 hr after Colcemid, the epidermal mitotic counts in dorsal skin were determined and the mitotic rates calculated. These were compared with the normal mitotic rates, and the ratios between the results from HU‐treated and ‐untreated animals were calculated. Hydroxyurea caused a considerable reduction in the mitotic rate with a trough at 6 hr, followed by a wave of increased mitotic rate with a peak at 14 hr, followed by a secondary drop at 20 hr, and then a return to normal. Another group of mice were given HU only, and the fraction of epidermal cells in G 2 was measured by flow cytometry. From these animals, without previous injection of Colcemid, we also determined the mitotic counts and calculated the mitotic durations. Cells piled up in G 2 for the first 6 hr after HU injection, then the G 2 compartment was emptied. the results are discussed in relation to previous results from this department showing the effect of the same dose of HU on DNA synthesis in the same mouse strain. It is concluded that HU not only blocks or retards DNA synthesis in epidermal cells, but also affects the movement of cells through G 2 and M. the cell kinetic effects of HU thus seem to be very complex.