Open AccessRate of Non‐Adherent Cell Loss From Long‐Term Cultures of Murine Bone Marrow: Effect of Medium Conditioned By the Adherent Cell Population
Author(s) -
Janssen W. E.,
Snell F. M.,
Anbar M.
Publication year - 1985
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1985.tb00704.x
Subject(s) - haematopoiesis , cell , population , progenitor cell , bone marrow , lysis , cell culture , biology , microbiology and biotechnology , cell division , immunology , andrology , chemistry , stem cell , medicine , biochemistry , genetics , environmental health
. The rate of random spontaneous cell loss from the non‐adherent cell population of long‐term cultures of murine bone marrow was determined. the measured rate of non‐adherent cell loss is affected by previous conditioning of the medium in which the non‐adherent cells are suspended. Specifically, the measured rate of non‐adherent cell loss is significantly slowed when the non‐adherent cells are suspended in medium conditioned in long‐term haematopoietic cultures instead of fresh medium. It appears that a subset of the adherent cell population is the source of the factor or factors within the medium which result in this slower measured rate of non‐adherent cell loss. This effect may be due to the stimulation to division of haematopoietic progenitor cells, offsetting the lysis of other non‐adherent cells.