Open AccessGraphical Method For Analysing Stathmokinetic Data
Author(s) -
Roti Joseph L.
Publication year - 1985
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1985.tb00697.x
Subject(s) - colcemid , ordinate , point (geometry) , perpendicular , doubling time , line (geometry) , mathematics , function (biology) , physics , cell culture , mathematical analysis , geometry , biology , microbiology and biotechnology , genetics
Abstract. The accumulation of cells in G 2 M measured flow cytometrically follows a straight line when plotted against time in colcemid with the transform ln (1 + fg 2 M), where f G 2 M, is the fraction of cells in the G 2 M peak. Extending this function to the value, In 2, will locate a point B. Drawing a line from point B to In 1 perpendicular to the abscissa, will locate point A at the initial value of In (1 + f G 2 M). A line from In 2 at t = O through point A will intersect the abscissa at the cell doubling time. Application of this method to the depletion of G 1 cells is also possible.