Open AccessA Novel Approach to the Study of Kinetically Changing Cell Populations
Author(s) -
Sheridan J. W.
Publication year - 1985
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1985.tb00672.x
Subject(s) - sedimentation , cell cycle , fractionation , thymidine , interpretation (philosophy) , labelling , biological system , cell , cell growth , microbiology and biotechnology , biology , exploit , chemistry , computational biology , computer science , biochemistry , chromatography , dna , paleontology , sediment , programming language , computer security
A method is described for estimating changes in cell cycle times during periods of rapid change in proliferation rate. This method, which depends upon the interpretation of pre‐ and post‐velocity sedimentation fractionation continuous thymidine labelling patterns, exploits the relationship between sedimentation rate and cell cycle location. By this means, cycle times can be estimated under conditions that are difficult (if not impossible) to analyse by FLM methods.