Open AccessSeparation By Velocity Sedimentation of Human Haemopoietic Precursors Forming Colonies in vivo and in vitro Cultures
Author(s) -
Niskanen E.,
Wells J. R.,
Golde D. W.,
Cline M. J.
Publication year - 1985
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1985.tb00670.x
Subject(s) - in vitro , in vivo , biology , sedimentation , microbiology and biotechnology , sedimentation coefficient , granulocyte , colony forming unit , chemistry , immunology , biochemistry , bacteria , paleontology , genetics , sediment , enzyme
Cells which give rise to granulocyte‐macrophage colonies under the influence of peripheral blood white cells (CFU‐c (WBC)) and Mo T cell conditioned medium (CFU‐c (Mo)) sedimented at a faster rate than the cells which form mixed erythroid‐granulocytic colonies in methylcellulose in vitro (CFU‐mix) and granulocytic (CFU‐dg) and megakaryocytic (CFU‐dm) colonies in diffusion chambers in mice. Despite identical peak sedimentation rate for the two CFU‐c populations, sedimentation profiles suggest that they are heterogeneous with respect to size. A proportion of CFU‐c (Mo) may be identical with CFU‐dg and CFU‐mix. Sedimentation profiles for cells which give rise to mixed colonies in vitro (CFU‐mix) and to granulocytic colonies in diffusion chambers in cyclophosphamide pretreated mice (CFU‐dg (CY)) and in Mo conditioned medium treated mice (CFU‐dg (Mo)) were similar. On the average CFU‐dm sedimented somewhat slower than CFU‐dg. These and other observations suggesting a close relationship between CFU‐dg and multipotential haemopoietic precursors are discussed.