Open AccessEstimation of Circadian Variations In Cell Cycle Phase Durations In Murine Epidermal Basal Cells
Author(s) -
Thorud Erik,
Aarnæs Erik,
Clausen Ole Petter F.,
Bjerknes Rolf,
Anker Peder
Publication year - 1985
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1985.tb00658.x
Subject(s) - circadian rhythm , basal (medicine) , biology , microbiology and biotechnology , cell cycle , cell , endocrinology , biochemistry , insulin
Circadian variations in the proliferative activity of squamous epithelia are well known. However, circadian variations in the duration of the various cell cycle phases (S, G 2 and mitosis) have been disputed. the percent labelled mitoses method, which is traditionally used to obtain duration of cell cycle phases, is poorly suited for identification of circadian variations. Therefore methods combining changes in compartment size (cell cycle phase) and cellular flux through the compartments have been used. Three different methods using such data are presented. These incorporate various simplifying assumptions that cause methodological errors. Limits for use of the different methods are indicated. the use of all three methods gives comparable and pronounced circadian variations in the duration of S and G 2 phase. These results are also compatible with circadian variations in the mitotic duration, but they may also represent artefacts due to sensitivity to model errors.