Open AccessDifferent Sensitivities of Granulocyte‐Macrophage and Erythroid Progenitor Cells of Normal Human Bone Marrow to S‐Phase‐Specific Agents
Author(s) -
Kubota Kazuo,
Preisler Harvey D.,
Costanzo Catherine
Publication year - 1984
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1984.tb00619.x
Subject(s) - bone marrow , progenitor cell , granulocyte , haematopoiesis , macrophage , thymidine , immunology , cfu gm , biology , stem cell , microbiology and biotechnology , chemistry , in vitro , biochemistry
The extraordinary sensitivity of early erythroid progenitor cells (BFU‐e) of normal human bone marrow to tritiated thymidine ([ 3 H]TdR) was studied. While exposure of bone‐marrow cells to [ 3 H]TdR for 1 hr resulted in the death of only 40% of the granulocyte‐macrophage progenitor cells (CFU‐c), 90% of BFU‐e were killed. Experiments in which normal bone‐marrow cells were mixed with bone‐marrow cells which had been exposed to [ 3 H]TdR demonstrated that the excessive killing of BFU‐e by [ 3 H]TdR reflected carry‐over of the [ 3 H]TdR by the exposed cells. A carry‐over effect was not observed for CFU‐c, suggesting the presence of a fundamental difference in the metabolism of TdR between CFU‐c and BFU‐e. There was a suggestion of a carry‐over effect regarding two other S‐phase‐specific agents, hydroxyurea and 1‐β‐D‐arabinofuranosylcytosine.