Open AccessThe Metaphase‐Arrest Technique Applied to Human Cervical Epithelium I.
Author(s) -
Ireland D.,
Monaghan J. M.
Publication year - 1984
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1984.tb00609.x
Subject(s) - epithelium , metaphase , andrology , cervical carcinoma , cervical intraepithelial neoplasia , in vivo , biology , pathology , medicine , cervical cancer , chromosome , genetics , cancer , gene
In order to study cell proliferation in intact human cervical epithelium, a technique involving metaphase arrest has been utilized. Metaphase accumulation is observed following intraepithelial administration of vincristine sulphate, at a predetermined optimum dose of 50 μg/ml. A significant delay before the onset of stathmokinetic activity is demonstrated; thereafter linearity of accumulation is apparent over a 4‐hr period following injection. The technique appears applicable to the estimation and comparison of cell production rates in vivo of normal cervical epithelium, cervical intraepithelial neoplasia, wart‐affected cervical epithelium and early invasive carcinoma. However, individual values are likely to be imprecise in isolation, indicating the need to study relatively large numbers of subjects in each group. Some practical difficulties are discussed.