Open AccessDelay In Entry Into S Phase After Heat Shock
Author(s) -
Dewey D. L.,
Holden J. L.
Publication year - 1984
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1984.tb00594.x
Subject(s) - thymidine , shock (circulatory) , dna synthesis , cell cycle , heat shock , thermal shock , microbiology and biotechnology , dna , biology , chemistry , heat shock protein , thermodynamics , cell , medicine , biochemistry , physics , gene
Synchronized cells of the Harding Passey melanoma grown in culture were given a heat shock treatment of 44° C for 36 min. Thymidine incorporation was measured at frequent intervals after heat shock to determine the time of onset of the next DNA synthetic period. If the heat shock was given at the end of G 1 , the following S was delayed by 20 hr. Heating at other times in the cell cycle resulted in an even longer interval before the onset of S. the end of G 1 was also the most resistant to hyperthermic killing and to the effect of heat on the magnitude of thymidine incorporation in the following S. Heating the cells a second time did not repeat the effect of the first treatment unless the second heat shock treatment was at a considerably higher temperature. Thus thermotolerance to heat shock killing also applies to cell‐cycle delay.