Open AccessCell Synchrony Techniques. II. Analysis of Cell Progression Data
Author(s) -
White R. Allen,
Grdina David J.,
Meistrich Marvin L.,
Meyn Raymond E.,
Johnson Tod S.
Publication year - 1984
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1984.tb00584.x
Subject(s) - cell , biology , computational biology , computer science , genetics
CHO cells which have been sorted by mitotic detachment, centrifugal elutriation and fluorescence activated cell sorting have been followed for up to 14 hr by flow cytometry to examine their progression characteristics. Mathematical modelling techniques were used to provide quantitative estimates of the cell‐cycle parameters. Mitotic detachment gives an 11.2‐hr cycle time with mean transit times T G1 , T s and T G2M equal to 3.2, 5.6 and 2.4 respectively. Cells prepared by central elutriation in an early G 1 state have a 14‐hr cycle time with T G1 , T s and T G2M of 5.7, 6.0 and 2.3 hr. Populations prepared by centrifugal elutriation enriched in early S and late S and G 2 M have transit times of 2.7, 5.9 and 1.6 hr and 4.9, 6.7 and 2.1 hr with cycle times of 11.2 and 13.2 hr respectively. Cell sorting for a G 1 population gives transit times of 9.8, 8.0 and 3.6 for an overall 21.4‐hr cycle time.