Open AccessSurvival and Repopulation of Irradiated ‘Pre‐Cfu‐C’ In Mice
Author(s) -
Aizawa Shin,
Amaki Ichita,
Miyanomae Takeshi,
Tsurusawa Masahito,
Mori Kazuhiro J.
Publication year - 1984
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1984.tb00576.x
Subject(s) - cfu gm , granulopoiesis , radiosensitivity , biology , bone marrow , progenitor cell , in vitro , colony forming unit , microbiology and biotechnology , in vivo , incubation , granulocyte , agar , macrophage , andrology , stem cell , immunology , irradiation , biochemistry , medicine , genetics , physics , bacteria , nuclear physics
Supernatants of murine bone‐marrow cultures contain a colony‐promoting factor (CPF) which increases the number of granulocyte and macrophage colonies in semi‐solid agar cultures in the presence of colony‐stimulating factor (CSF). Incubation of bone‐marrow cells with CPF results in an increase in the number of granulocyte/macrophage progenitor cells (CFU‐c) and the CPF‐responsive cells may be younger than the CFU‐c. We have investigated the radiosensitivity and the pattern of the recovery after irradiation of CPF‐responsive cells. We found that the radiosensitivity of CPF‐responsive cells was significantly lower than those of CFU‐c. burst‐forming units‐erythroid (BFU‐e) and pluripotent stem cells in vivo (CFU‐s) and in vitro (CFU‐mix). the CPF‐responsive cells remained subnormal even at 28 days after irradiation of the mice, a time when the CFU‐s and CFU‐c had recovered completely. Therefore the CPF‐responsive cells may constitute a separate compartment, namely ‘pre‐CFU‐c’, in the maturation sequence of granulopoiesis, and this maturation of the ‘pre‐CFU‐c’ to CFU‐c seems to be highly stimulated after irradiation to counterbalance the influx from CFU‐s.