Open AccessAnalysis of X‐Ray‐Induced Cell‐Cycle Perturbations In Mouse Osteosarcoma Cells: A Two‐Signal Cell‐Cycle Model
Author(s) -
Meeteren A.,
Wijk R.,
Stap J.,
Deys B. F.
Publication year - 1984
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1984.tb00573.x
Subject(s) - cell cycle , cell , cell growth , cell division , osteosarcoma , biology , irradiation , microbiology and biotechnology , andrology , cancer research , genetics , medicine , physics , nuclear physics
The effects of X‐irradiation on mouse osteosarcoma cells have been studied by time‐lapse cinematography and the resulting pedigrees have been analysed statistically. It is shown that the irradiation treatment causes three types of cell kinetic lesions: cell death (disintegration), cell sterilization (failure to divide) and proliferation delay. the first two lesions are the most important with regard to survival of the irradiated cell in a clonal assay. of these two lesions, sterilization appears to be highly correlated for sister cells, while this is not true for cell disintegration. This indicates that cell survival in a clonal assay may be a function of the ratio of the incidences of these two types of lesions. the X‐ray‐induced proliferation delay was studied in terms of intermitotic time distributions, mother‐daughter correlation and sibling correlation in relation to the current cell‐cycle phase at the time of treatment. This analysis shows that the effects of irradiation on these cell‐cycle characteristics is highly cellcycle‐dependent. A qualitative model to account for the observations is presented.