Open AccessA Convenient Source of Cfu‐S Inhibitors: the Fetal Calf Liver *
Author(s) -
Guigon M.,
WdzieczakBakala J.,
Mary J. Y.,
Lenfant M.
Publication year - 1984
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1984.tb00567.x
Subject(s) - fetus , biology , chemistry , pregnancy , genetics
The presence of a bone‐marrow stem‐cell inhibitor able to prevent CFU‐s entry into DNA synthesis after cytosine arabinoside (Ara‐C) treatment has been detected in 7‐month‐old fetal calf liver. the inhibitory fraction was obtained through ultrafiltration of a delipidated tissue extract powder and purified by BioGel‐P‐2 chromatography. the elution pattern on Sephadex G10 is similar to that of the bone‐marrow inhibitory extract previously obtained from fetal calf bone marrow. It corresponds to a low‐molecular‐weight molecule (MW > 2000), devoid of species specificity and having no inhibitory effect on GM‐CFC proliferation.