Open AccessRadiotoxicity of incorporated [ 3 H]thymidine Consequences for the interpretation of FLM data of irradiated cell populations
Author(s) -
Beck H.P.
Publication year - 1982
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1982.tb01569.x
Subject(s) - thymidine , irradiation , radiochemistry , mitosis , mitotic index , cell , in vitro , nuclear medicine , chemistry , biology , biochemistry , genetics , physics , medicine , nuclear physics
. Problems in the interpretation of FLM data of externally irradiated cell populations are mainly due to the interference of radiation effects with radiotoxic effects originating from incorporated [ 3 H]thymidine. These problems were investigated using L‐929 cells flash labelled in vitro with [ 3 H]thymidine (30 min, 0.3 μCi/ml, 40 Ci/mM) and irradiated with 2 Gy of 200 kV X‐rays; the fractions of labelled mitoses and the index of labelled and of unlabelled mitoses were determined. The results showed that the FLM is not an adequate parameter to quantify the early cell kinetic changes in irradiated cell populations.