Open AccessStem cell (CFU‐s) proliferation in sublethally irradiated mice
Author(s) -
Nečas E.
Publication year - 1982
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1982.tb01073.x
Subject(s) - irradiation , cell division , thymidine , cell growth , dna synthesis , biology , stem cell , microbiology and biotechnology , period (music) , andrology , cell , dna , immunology , biochemistry , medicine , physics , nuclear physics , acoustics
. The proliferation rate of haemopoietic stem cells (CFU‐s) was followed after sublethal total body irradiation with 1.5 Gy. The [ 3 H]‐thymidine suicide technique was used to measure the CFU‐s proliferation rate. The measurements extended from 10 min after irradiation up to 21 days. The CFU‐s did not enter the DNA synthesis period (S‐phase) shortly after irradiation, as had been previously suggested, but did so only with a delay of 14–16 hr. A large scatter of results was explained by an oscillatory pattern in CFU‐s proliferation. The CFU‐s prepared for cell division in synchronized waves, with a period of 20–22 hr.