Open AccessAnalysis of regenerating hepatic cell replication in vivo by differential chromatid staining
Author(s) -
Kato H.,
Reff M.,
Schneider E. L.
Publication year - 1982
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1982.tb01069.x
Subject(s) - cell cycle , bromodeoxyuridine , differential staining , in vivo , chromatid , staining , metaphase , cell , microbiology and biotechnology , biology , chemistry , chromosome , cell growth , biochemistry , genetics , gene
. The cell cycle of mouse hepatic cells was examined in vivo following partial hepatectomy, by differential chromatid staining in the presence of non‐inhibitory concentrations of bromodeoxyuridine (BrdU). Using this technique, distribution curves were obtained for the appearance of metaphase cells in successive generations, and mean cell cycle time (11 hr) was determined. Cell cycle times derived with this technique are several‐fold faster than previous reports of regenerating liver which used radionucleotide labelling.