Open AccessSubclassification of cells in S phase in a partially synchronized cell system
Author(s) -
Møller U.,
Keiding N.,
Engel F.
Publication year - 1982
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1982.tb01034.x
Subject(s) - flow cytometry , circadian rhythm , dna , dna synthesis , intracellular , microbiology and biotechnology , phase (matter) , biology , biophysics , chemistry , genetics , endocrinology , organic chemistry
. A circadian dependent delay in the incorporation of [ 3 H]TdR into DNA, presumably due to variations in the intracellular pool of [ 3 H]TdR derivatives, was found. It seems reasonable to relate this effect to a circadianally varying age distribution of cells in S phase. At any given time the S phase cells showed large variations in DNA synthesis rate, but it was still possible to identify a mean diurnal variation in the DNA synthesis rate. Differences in the ability of S phase cells to incorporate [ 3 H]TdR are also discussed in relation to flow cytometrical measurements, and this contributes to the understanding of the commonly observed phenomenon that flow cytometry estimates of S‐fractions are higher than those obtained with autoradiography.