Open AccessRadiosensitivity variation during the cell cycle in pronuclear mouse embryos in vitro
Author(s) -
Domon M.
Publication year - 1982
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1982.tb01027.x
Subject(s) - radiosensitivity , embryo , blastomere , blastocyst , biology , andrology , cell cycle , cleavage (geology) , in vitro , microbiology and biotechnology , cell , embryogenesis , genetics , medicine , radiation therapy , paleontology , fracture (geology)
. The radiosensitivity of pronuclear mouse (B6D2 F1 x ICR) embryos has been measured in vitro as a function of time during the cell cycle. This was done by measuring the dose of X‐rays (LD50) required to prevent development of 50% of the pronuclear embryos to the blastocyst stage in 5 days of culture. The LD50 was found to vary from 1 to 2 Gy during the period from G1 to the first cleavage. The cell cycle in the pronuclear embryo was analysed by [ 3 H]thymidine autoradiography. Compared with earlier studies on two‐cell mouse embryo radiosensitivity, the pronuclear embryos appear to be more sensitive to radiation than the two‐cell embryos. If, however, one considers the radiation sensitivity on a blastomere basis, the pronuclear embryos are not different in their radiation sensitivity from the two‐cell embryos. Thus, during the early cleavage stages of mice, radiosensitivity is mainly governed by the content of cells of various cell cycle ages in the embryo.