Open AccessPartial purification of a low molecular weight inhibitor of epidermal DNA synthesis
Author(s) -
Quirk A. V.,
Pain R. H.,
Davison Maureen,
Shuster S.
Publication year - 1982
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1982.tb01021.x
Subject(s) - dna synthesis , epidermis (zoology) , silicic acid , thymidine , epidermal growth factor , dna , chemistry , biochemistry , extraction (chemistry) , ethyl acetate , ethanol , absorption (acoustics) , cell growth , in vitro , biology , chromatography , organic chemistry , physics , receptor , anatomy , acoustics
. An attempt has been made to purify factors present in aqueous extracts of pig epidermis which inhibit epidermal cell proliferation. A lipophilic factor of low molecular weight (less than 10,000), has been shown to inhibit DNA synthesis as measured by the uptake of tritiated thymidine in mouse ear epidermis. Purification by alcohol precipitation, ethyl acetate extraction and silicic acid column chromatography produced a fifteen‐fold increase in the specific activity of the inhibitory action. It seems likely that aggregation or absorption of this low molecular weight factor may explain the high molecular weight of epidermal cell proliferation inhibitors previously studied, as well as the difficulty in their characterization.