Open AccessPresence of pluripotent haemopoietic precursors in vitro (CFU‐mix) in haemopoietic tissues from mice of W/Wv genotype
Author(s) -
Hara H.,
Ohe Y.,
Noguchi K.,
Nagai K.,
Tsuyama K.,
Kitamura Y.
Publication year - 1982
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1982.tb01020.x
Subject(s) - haematopoiesis , in vitro , biology , spleen , bone marrow , congenic , in vivo , microbiology and biotechnology , immunology , stem cell , genetics , gene
. The presence or absence of haemopoietic precursors, which produce mixed colonies in vitro (CFU‐mix) was examined in the bone marrow and spleen of (WB x C57BL/6) F1‐ W/W v mice. Despite the failure of macroscopically evident colonyformation in the spleens of irradiated mice, haemopoietic cells of W/W v mice did produce macroscopically‐evident mixed colonies containing erythroid cells, macrophages, and often megakaryocytes, in culture medium. The size and constitution of mixed colonies derived from W/W v mice were comparable to those of mixed colonies from congenic +/+ mice. The present results appear consistent with in vivo haemopoiesis in the W/W v mice, which is obviously deficient, but sufficient for survival.