Open AccessEFFECT OF TRITIATED THYMIDINE ON THE KINETICS AND VIABILITY OF 9L CELLS IN VITRO
Author(s) -
Wiezsaecker M.,
Hoshino T.,
Kobayashi S.
Publication year - 1981
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1981.tb00562.x
Subject(s) - thymidine , incubation , petri dish , in vitro , microbiology and biotechnology , biology , cell , incubation period , andrology , chemistry , biochemistry , medicine
The colony‐forming efficiency of 9L rat gliosarcoma cells was unaffected by treatment with 0.1 μCi/ml of [ 3 H]TdR. However, when cells were treated with 1 or 10 μCi/ml of [ 3 H]Tdr, cell growth was reduced and cell survival decreased. When monolayer 9L cells were treated with 1 μCi/ml of [ 3 H]TdR for up to 72 hr, approximately 5% survived, which is closely related to the percentage of non‐cycling cells in this system. When cells were treated with 10 μCi/ml of [ 3 H]TdR for 72 hr, less survival was observed. the additional cell kill observed may be induced by [ 3 H]TdR released from doomed cells into petri dishes during the incubation period of the colony‐formation assay.