Open AccessProliferation In Liquid Culture of Myeloid Progenitor Cells From the Blood of Normals and Patients With Aplastic Anaemia
Author(s) -
Fitchen J. H.,
Deregnaucourt Josette,
Cline M. J.
Publication year - 1981
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1981.tb00561.x
Subject(s) - progenitor cell , myeloid , immunology , progenitor , myeloid cells , haematopoiesis , biology , medicine , stem cell , microbiology and biotechnology
We studied myeloid progenitor cells (CFUc) from peripheral blood mononuclear leukocytes (MNL) of normals and patients with severe aplastic anaemia (AA). Baseline CFUc averaged 4.4 φ 1.5 (range 0–17)/10 6 MNL in fifteen normals and 0 φ 0/10 6 MNL in six patients with severe AA ( P > 0.05). to assess CFUc proliferative capacity, 10 7 MNL were put into liquid culture in Marbrook chambers with colony‐stimulating activity and sub‐cultured in agar at intervals up to 10 days. CFUc from normal MNL increased from 44 φ 15/chamber at Day 0 to 156 φ 33/chamber at peak value ( P > 0.02). In contrast, CFUc from AA MNL remained undetectable throughout the period of liquid culture (AA peak v. normal peak; P > 0.001). These results indicate a marked decrease in circulating CFUc in patients with severe AA, and a profound abnormality in CFUc proliferation in vitro. This abnormality could be due to defective replication of CFUc, a lack of feed‐in from more primitive precursors, or both.