Open AccessLaser Flow Cytofluorometric Analysis of Htc Cells Synchronized With Hydroxyurea, Nocodazole and Aphidicolin
Author(s) -
MillerFaurès A.,
Michel N.,
Aguilera A.,
Blave A.,
Miller A. O. A.
Publication year - 1981
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1981.tb00556.x
Subject(s) - nocodazole , aphidicolin , colcemid , metaphase , flow cytometry , cell cycle , biology , microbiology and biotechnology , chemistry , cell , mitosis , chromosome , genetics , gene , cytoskeleton
The technique of laser flow cytofluorometry has been used to monitor the arrival in G 1 and the subsequent progression through the cell cycle of HTC cells accumulated in metaphase with colcemid alone or after treatment with hydroxyurea and Nocodazole. Under the experimental conditions used in this study, the latter procedure gives much better results, avoiding in particular the extensive formation of micronucleated cells. Aphidicolin, an inhibitor of DNA polymerase, in combination with Nocodazole, provides a useful method to tightly synchronize these cells at the G 1 /S border.