Open AccessClonal Growth of Leukaemic Cells In Vitro
Author(s) -
Preisler H. D.,
Epstein J.
Publication year - 1981
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1981.tb00546.x
Subject(s) - clone (java method) , agar , liquid culture , in vitro , biology , liquid medium , cell growth , cell culture , agar plate , microbiology and biotechnology , chemistry , dna , biochemistry , genetics , bacteria , chromatography , botany
Human leukaemic cell specimens were obtained from patients and directly plated into soft agar ( t = 0) or cultured for 1 week in liquid phase and then plated in soft agar. Growth for 1 week in liquid phase allowed the clonal growth in agar of leukaemic specimens which were unable to clone at t = 0. Clonal growth after liquid culture consisted of the usual leukaemic type of cluster‐colonies, growth of a new type of ‘syncytial’ cell colony or a mixture of colony types. In addition, marrow from a patient with acute lymphocytic leukaemia produced normal‐appearing colonies after 1 week of growth in liquid phase. These studies suggest a similarity in the growth requirements of some leukaemic cells and normal CFUd cells.