Open AccessA Flow Cytometric In Vivo Chalone Assay Using Retransplanted Old Murine Jb‐1 Ascites Tumour Cells
Author(s) -
Barfod N. M.
Publication year - 1981
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1981.tb00545.x
Subject(s) - in vivo , ascites , ascitic fluid , transplantation , flow cytometry , in vitro , dna synthesis , ratón , chemistry , pathology , biology , microbiology and biotechnology , cancer research , immunology , medicine , biochemistry
A flow cytometric in vivo chalone assay is described. Transplantation of old JB‐1 ascites tumour cells to new hosts induced an influx of tumour cells, with G 1 DNA content, to the S phase. This induction could be reversibly and specifically blocked by injections of an ultrafiltrate of old JB‐1 ascites fluid. The method described is superior to a previously published in vivo chalone assay using regenerating ascites tumours. Owing to a reduced variability in time of onset of DNA synthesis, a smaller scatter of observations is achieved and thus the number of mice per group may be reduced using the new method. In contrast to the older technique, the present one does not necessitate killing of mice during the observation period.