Open AccessPost‐Irradiation Thymocyte Regeneration After Bone Marrow Transplantation Ii. Physical Characteristics of Thymocyte Progenitor Cells
Author(s) -
Boersma W. J. A.,
Daculsi R.,
Westen G.
Publication year - 1981
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1981.tb00523.x
Subject(s) - thymocyte , bone marrow , progenitor cell , transplantation , biology , immunology , buoyant density , population , regeneration (biology) , cell , microbiology and biotechnology , stem cell , andrology , t cell , medicine , biochemistry , immune system , virus , environmental health
Bone marrow cells were separated according to buoyant density, velocity sedimentation and cell surface charge. Fractionated (C3H × AKR)F 1 bone marrow cells were transplanted into lethally‐irradiated C3H recipients. In all fractions, the CFUs content and the capacity to restore the thymus cell population were determined. For all the physical parameters tested, thymocyte progenitor cells show the same distribution as CFUs. the relationship between number of thymocyte progenitor cells and number of CFUs is dependent on density. Bone marrow progenitors of PHA responsive cells are of low buoyant density and show a distribution which resembles the distribution of the progenitors of Thy 1 positive cells. After transplantation of large numbers of bone marrow cells into irradiated mice, no significant change in the CFUs content of the thymus was observed.