Open AccessRADIOTOXICITY OF INCORPORATED [ 3 H]THYMIDINE AS STUDIED BY AUTORADIOGRAPHY AND FLOW CYTOMETRY CONSEQUENCES FOR THE INTERPRETATION OF FLM DATA
Author(s) -
Beck H.P.
Publication year - 1981
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1981.tb00521.x
Subject(s) - mitosis , thymidine , flow cytometry , kinetics , in vitro , chemistry , biology , microbiology and biotechnology , biophysics , biochemistry , physics , quantum mechanics
The radiotoxic effects of incorporated [ 3 H]thymidine on proliferation kinetics of flash‐labelled (30 min, 0.3 μCi/ml, 40 Ci/mM) L‐929 cells in vitro were studied by means of autoradiography and flow cytometry. the flow cytometric results obtained by applying the BUdR‐33258 Hoechst technique, using new evaluation procedures, showed that the labelled cells are delayed in their progression through the S and G 2 + M phases, leading to mitotic delay. From autoradiographs, the fraction of labelled mitoses was determined and, in addition, the ratio of labelled and of unlabelled mitotic cells to all cells. the radiotoxic effects are not evident from the FLM curve, even if the ratio of labelled mitotic cells to all cells shows a highly distorted shape. A mathematical model has been developed that describes the perturbed cell kinetics due to radiotoxic effects of the incorporated [ 3 H]thymidine. These findings have considerable consequences for the interpretation of autoradiograhic data, especially of labelled mitoses curves.