Open AccessPROTECTION OF CYCLING CFUs AGAINST HYDROXYUREA BY LOW DOSES OF ACTINOMYCIN D
Author(s) -
Fehér I.,
Gidali Julia
Publication year - 1980
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1980.tb00480.x
Subject(s) - in vivo , in vitro , thymidine , population , biology , ehrlich ascites , stem cell , microbiology and biotechnology , andrology , pharmacology , chemistry , biochemistry , cancer research , medicine , tumor cells , environmental health
Low dose (80 μg/kg) Actinomycin D (AD) produced a significant but transient inhibition of proliferation of the haemopoietic stem cells (CFUs) in chimaeras or in mice regenerating after sublethal irradiation. The same dose of AD had no effect on the resting CFUs population. During the period of proliferation inhibition, CFUs proved to be insensitive to the killing effect of [ 3 H]thymidine in vitro and hydroxyurea (HU) in vivo. In Ehrlich ascites tumour (EAT) bearing mice enhanced CFUs turnover rate was found. Eighty μg/kg AD produced a selective effect in these mice: it protected the proliferating CFUs population without diminishing the effect of hydroxyurea on the tumour cells.