Open AccessTHE PROLIFERATION KINETICS OF NHIK 1922 CELLS IN VITRO AND IN SOLID TUMOURS IN ATHYMIC MICE
Author(s) -
Rofstad E. K.,
Pettersen E. O.,
Lindmo T.,
Oftebro R.
Publication year - 1980
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1980.tb00459.x
Subject(s) - in vivo , in vitro , kinetics , mitosis , biology , microbiology and biotechnology , growth curve (statistics) , cell division , flow cytometry , chemistry , andrology , cell , biochemistry , genetics , medicine , physics , quantum mechanics , economics , econometrics
The proliferation kinetics of cells of the line NHIK 1922 grown in vitro and as solid tumours in the athymic mutant nude mouse has been studied. In vitro , growth curves were determined for exponentially growing populations and for populations synchronized by mitotic selection. The phase durations for these populations were determined by flow cytofluorometric measurements of DNA‐histograms and pulsed incorporation of [ 3 H]TdR respectively. The generation time and the phase durations for synchronized populations were found to be about equal to those for exponentially growing populations. The duration of the phases G 1 , S and G 2 + M was found to be 8·5–9·5, 11·0–12·0 and 6·0–6·5 hr respectively, i.e. the generation time was 26·5–27·0 hr. The proliferation kinetics in vivo were studied by flow cytofluorometry and by the technique of percentage labelled mitoses. The median duration of S‐phase and (G 2 + M)‐phase in vivo was found to be approximately the same as that observed in vitro , while the median duration of G 1 ‐phase was found to be approximately 5 hr longer in vivo than under the present in vitro growth conditions. The growth fraction in vivo was estimated to be approximately 50%. The non‐proliferative compartment of the tumour cells was found to consist mainly of cells with the DNA‐content of cells in G 1 ‐phase. It is concluded that the reduced rate of proliferation of NHIK 1922 cells in vivo is correlated with alterations in the duration of G 1 ‐phase and, hence, the proportion of cells in G 1 ‐phase.