Open AccessKINETICS OF CELL PROLIFERATION IN THE PRE‐IMPLANTED MOUSE EMBRYO IN VIVO AND IN VITRO
Author(s) -
Streffer C.,
Beuningen D.,
Molls M.,
Zamboglou N.,
Schulz S.
Publication year - 1980
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1980.tb00456.x
Subject(s) - in vivo , embryo , mitotic index , mitosis , cell growth , in vitro , cell cycle , biology , andrology , microbiology and biotechnology , cell division , cell , dna synthesis , biochemistry , genetics , medicine
The cell proliferation of pre‐implanted mouse embryos was investigated after development in vivo and in vitro . The studies were started at the pronuclear stage, 2 h post conception (p.c.) and continued until the hatching of blastocysts, 120–144 h p.c. The number of cell nuclei, the DNA content of each nucleus, the mitotic index and the labelling index were determined. From these data it was possible to calculate the length of the cell generation cycle and its various phases. With the exception of the first cell cycle the S‐phase was constant. The G 1 ‐ as well as the G 2 ‐phase varied in length during the different cell cycles. From 31–72 h p.c. the increase in cell number was exponential. After cultivation in vitro this increase was smaller than in vivo . At later periods the proliferation rate decreased with proceeding development. In late blastocysts most of the cells were in the G 1 ‐phase. The development of the embryos was somewhat faster in vivo than in vitro . But in principle conditions were comparable.