Open AccessCELL‐PRODUCTION RATES ESTIMATED BY THE USE OF VINCRISTINE SULPHATE AND FLOW CYTOMETRY I. AN IN VITRO STUDY USING MURINE TUMOUR CELL LINES
Author(s) -
Barfod I. H.,
Barfod N. M.
Publication year - 1980
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1980.tb00444.x
Subject(s) - flow cytometry , in vitro , vincristine , cell culture , cancer research , cell , biology , immunology , chemistry , microbiology and biotechnology , pharmacology , biochemistry , chemotherapy , genetics , cyclophosphamide
A method for the evaluation of cell‐production rates is described which combines flow cytometry (FCM) and the stathmokinetic method. By means of FCM it is possible to estimate the distribution of cells with G 1 , S and (G 2 + M) DNA content in a population. As this method gives the relative (G 2 + M) DNA content of cells within the cell cycle, it may be possible to evaluate cell‐production rates by this technique. In the present study it was found that administration of a metaphase‐arresting (stathmokinetic) agent, vincristine sulphate (VS), to asynchronous cell populations of three different murine tumour cell lines in vitro increased the peak representing cells with (G 2 + M) DNA content as the number of mitotic (M) cells increased during the period of treatment. The accumulation of mitotic cells was determined by cell counts on smears under the microscope and compared with the increase in the (G 2 + M) DNA peak measured by FCM as a function of time after the administration of VS. Good agreement was obtained between the cell‐production rates as estimated by FCM and by mitotic counts in all three cell lines investigated.