Open AccessALTERATION OF NEONATAL RAT PAROTID GLAND ACINAR CELL PROLIFERATION BY GUANETHIDINE‐INDUCED SYMPATHECTOMY
Author(s) -
Klein R. M.
Publication year - 1979
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1979.tb00164.x
Subject(s) - guanethidine , sympathectomy , parotid gland , acinus , amylase , anatomy , salivary gland , endocrinology , medicine , microbiology and biotechnology , biology , chemistry , pathology , pancreas , biochemistry , enzyme , stimulation
Newborn rats were injected with guanethidine‐sulfate (20 μg/g body weight) every 48 hr from 12 hr after birth until day 14 (eight injections per animal). The guanethidine treatment resulted in an 86% absolute reduction in cell number in the superior cervical ganglia of 15 day old rats. The cells which remained after guanethidine treatment showed destruction of mitochondria and an extensive decrease in endoplasmic reticulum. Chemical sympathectomy with guanethidine induced a 3.1 hr lengthening of the acinar cell generation cycle time (17.4 hr to 20.5 hr), resulting from a longer G 1 period (6.9 hr in the control group as compared to 10.5 hr in the guanethidine‐treated group), as well as a decrease in the mean percentage of [ 3 H]thymidine‐labeled acinar cells (22.3 ± 0.5% to 19.3 ± 0.5%) and mean acinar cell mitotic index (2.6 ± 0.2% to 2.1 ± 0.1%). A circadian rhythm was found to exist in parotid gland acinar cell mitotic activity of 15 day old rats and the amplitude of the rhythm was reduced from 26.5% to 14.9% in guanethidine‐treated rats. This study indicates that the diminution of sympathetic influence on the developing parotid gland results in a slight, but significant alteration in acinar cell proliferation.