Open AccessINTERFERENCE OF SEX‐RELATED FACTORS IN THE RESPONSE OF LIVER CELLS TO EXPERIMENTAL MITOTIC STIMULI
Author(s) -
Lombard M.N.,
Nadal C.,
FiszerSzafarz B.,
Rumeur E. Le,
Zajdela F.
Publication year - 1979
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1979.tb00161.x
Subject(s) - castration , ovariectomized rat , medicine , endocrinology , hepatectomy , stimulation , biology , hepatocyte , mitosis , hormone , ovary , andrology , resection , in vitro , surgery , biochemistry , microbiology and biotechnology
Stimulation of liver cell multiplication was obtained under two different experimental conditions.1 A single injection of casein solution resulted in (a) an identical synchronized mitotic wave response in 10‐day old male and female rats and (b) a significantly lower response in adult male rats compared to females, a difference which was reduced by castration of males at birth but essentially maintained if animals were operated when 10 days old. 2 Partial hepatectomy shortly after puberty resulted in active hepatocyte multiplication occurring 3 hr earlier in females than in males. This difference was suppressed when females were ovariectomized at birth and significantly reduced when they were spayed at a later age. Hepatocytes of castrated females entered actively into S phase 2 hr later than the sham‐operated controls. Unilateral ovariectomy on the other hand indicated that during compensatory and/or hyper‐compensatory activity of the single ovary there was a maximum difference between the male and female rate of [ 3 H]thymidine uptake in liver nuclei 20 hr after hepatectomy. A further kinetic study ( t = 25, 30, 40, 65, 90 hr) indicated no significant sex‐related difference in the number of S phases per 10,000 cells.The DNA content of regenerating versus control livers was comparable in both sexes at t = 22 and 90 hr but higher in females at t = 40 and 65 hr. A possible early postnatal interference of certain hormonal mechanisms in the receptivity to mitotic stimuli is postulated and discussed.