Open AccessNON‐RANDOM ASSOCIATION OF MITOTIC CELLS FROM CULTURED HUMAN LYMPHOCYTE SUSPENSIONS
Author(s) -
Fitzgerald P. H.
Publication year - 1979
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1979.tb00158.x
Subject(s) - metaphase , mitosis , pairing , biology , lymphocyte , microbiology and biotechnology , cell , chromosome , immunology , genetics , physics , gene , superconductivity , quantum mechanics
ABSTRACT By using chromosome markers in two separate cell lines of a human dispermic chimera, it was shown that 4.9% of metaphases in suspensions of PHA‐cultured lymphocytes were paired because of non‐random factors. A similar amount of metaphase pairing occurred in cultured lymphocytes of normal donors, and evidence from the relative mitotic cycles of the paired cells indicated that some of this pairing was non‐random. Such non‐random pairing could be a source of bias in cell kinetic and other studies involving metaphase cells in lymphocyte cultures.