Open AccessTHE RELATIONSHIP BETWEEN STEM CELL SEEDING EFFICIENCY AND POSITION IN CELL CYCLE
Author(s) -
Monette F. C.,
DeMello J. B.
Publication year - 1979
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1979.tb00123.x
Subject(s) - seeding , cycling , spleen , cell cycle , biology , stem cell , cell , colony forming unit , cell division , microbiology and biotechnology , immunology , biochemistry , genetics , agronomy , archaeology , bacteria , history
The seeding efficiency of colony‐forming cells from normal, regenerating and velocity‐sedimented cycling and non‐cycling narrow preparations was compared. Colony‐forming cells in cycle were found to exhibit a 50% reduction in splenic seeding when compared to normal marrow or sedimented non‐cycling cells. The results of this study indicate that the spleen colony assay underestimates the total number of colony‐forming cells by a fraction which is directly related to the number of cells in cycle.