Open AccessA SIMPLE METHOD FOR PRODUCING DIFFERENT GROWTH FRACTIONS IN VITRO FOR USE IN ANTI‐CANCER DRUG STUDIES
Author(s) -
Barranco S. C.,
Bolton W. E.,
Novak J. K.
Publication year - 1979
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1979.tb00109.x
Subject(s) - in vitro , drug , simple (philosophy) , pharmacology , biology , biochemistry , philosophy , epistemology
A simple technique has been used experimentally to produce in vitro Chinese hamster ovary cells with growth fractions ranging from 0 to 100%. Known numbers of exponentially growing and plateau‐phase tissue culture cells were mixed in various proportions to yield the desired final growth fraction. Cells attach to the culture flask surface within 1 hr of mixing. Treatment at that time with the nitrosourea compounds, CCNU and MeCCNU, resulted in differential drug survival sensitivities that were dependent upon the growth fraction of the population treated.