Open AccessON THE EXISTENCE OF ‘ARRESTED G 2 CELLS’ IN MOUSE EPIDERMIS
Author(s) -
Sauerborn R.,
Balmain A.,
Goerttler K.,
Stohr M.
Publication year - 1978
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1978.tb00896.x
Subject(s) - epidermis (zoology) , mitosis , population , microbiology and biotechnology , biology , cell , function (biology) , compartment (ship) , chemistry , anatomy , genetics , demography , sociology , oceanography , geology
It has been postulated that mouse epidermis contains two populations of resting cells, one of which is blocked at the G 1 ‐S boundary and the other between G 2 and mitosis. the ‘arrested G 2 cells’ were estimated, by the labelled mitosis method, to comprise 510% of the epidermal population and presumed to function as a ‘reserve pool’ which could be activated by wounding. A comprehensive search has now been carried out for arrested G 2 cells in mouse epidermis using the direct methods of single cell and flow through cytophotometry. No evidence was obtained which supports the existence of such a cell compartment. Suitable control experiments were carried out to ensure that G 2 cells were not lost during the isolation of epidermal nuclei.