Open AccessGrowth Fraction and Cycle Duration of Hepatocytes In the Three‐Week‐Old Rat
Author(s) -
Schultze B.,
Kellerer A. M.,
Grossmann C.,
Maurer W.
Publication year - 1978
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1978.tb00892.x
Subject(s) - mitosis , doubling time , cell cycle , andrology , analysis of variance , fraction (chemistry) , biology , medicine , endocrinology , chemistry , cell , microbiology and biotechnology , biochemistry , chromatography
The proliferation of hepatocytes in the liver of 3‐week‐old rats has been investigated by autoradiographic methods. This investigation is a continuation of earlier work on the same topic (Schultze & Maurer, 1972; 1973). 21 days after birth, 102 rats received a single injection of 3 H‐TdR. the percentage of labelled mitoses was then determined 1 hr later and at various times throughout the interval up to 12 days after application of 3 H‐TdR. In agreement with earlier work, a first peak of labelled mitoses was found 7 hr after 3 H‐TdR injection. the area under the peak indicates an S phase duration of 8 hr. In addition a second very broad peak of labelled mitoses was found between 2 and 12 days after pulse labelling. the analysis of the results leads to the conclusion that the hepatocytes of the 3‐week‐old rat have a growth fraction close to 1 and a doubling time of 6–7 days. This is at variance with earlier results of Post, Huang & Hoffman (1963) and Grisham (1969) who had derived a value of 21.5 hr for the duration of the cell cycle and a value of only 0. 1–0.2 for the growth fraction of the hepatocytes.