Open AccessANALYSIS OF GROWTH OF TETRAPLOID NUCLEI IN ROOTS OF VICIA FABA
Author(s) -
Bansal J.,
Davidson D.
Publication year - 1978
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1978.tb00886.x
Subject(s) - interphase , vicia faba , prophase , cell cycle , biology , ploidy , population , colchicine , mitosis , microbiology and biotechnology , cell division , vicia , cell nucleus , cell , botany , nucleus , meiosis , genetics , demography , sociology , gene
Growth of nuclei of a marked population of cells was determined from G 1 to prophase in roots of Vicia faba. the cells were marked by inducing them to become tetraploid by treatment with 0.002% colchicine for 1 hr. Variation in nuclear volume is large; it is established in early G 1 and maintained through interphase and into prophase. One consequence of this variation is that there is considerable overlap between volumes of nuclei of different ages in the cell cycle; nuclear volume, we suggest, cannot be used as an accurate indicator of the age of the cell in its growth cycle. Nuclei exhibit considerable variation in their growth rate through the cell cycle. of the marked population of cells, about 65% had completed a cell cycle 14–15 hr after they were formed. These tetraploid nuclei have a cell cycle duration similar to that of fast cycling diploid cells of the same roots. Since they do complete a cell cycle, at least 65% of the nuclei studied must come from rapidly proliferating cells, showing that variability in nuclear volumes must be present in growing cells and cannot be attributed solely to the presence, in our samples, of non‐cycling cells.