Open AccessA New Analytical Method For Determining Duration of Phases, Rate of Dna Synthesis and Degree of Synchronization From Flow‐Cytometric Data On Synchronized Cell Populations
Author(s) -
BECK H.P.
Publication year - 1978
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1978.tb00882.x
Subject(s) - cell cycle , synchronization (alternating current) , flow cytometry , mitosis , degree (music) , biological system , dna synthesis , cell , dna , flow (mathematics) , cell synchronization , biology , phase (matter) , microbiology and biotechnology , chemistry , mathematics , physics , genetics , mechanics , topology (electrical circuits) , combinatorics , organic chemistry , acoustics
L‐cells synchronized by mitotic selection were investigated by flow‐cytometry nd the fractions of cells in the various cell cycle compartments were determined as a function of time. A new analytical evaluation procedure was developed, by which the mean transit‐times of cells through various cell cycle phases can be calculated from these data. Three examples for application of the method are presented: (1) determination of the duration of G 1 , S, G 2 + M and of the whole cell cycle; (2) calculation of the rate of DNA synthesis in several subcompartments of the S‐phase; and (3) evaluation of the degree of synchronization at different stages of the cell cycle.