Open AccessQUANTIFICATION OF ENTRY INTO AND EXIT FROM THE CELL CYCLE IN HUMAN LYMPHOCYTE CULTURES
Author(s) -
Dorian R.,
Bernheim J. L.,
Mendelsohn J.
Publication year - 1978
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1978.tb00873.x
Subject(s) - thymidine , lymphocyte , compartment (ship) , biology , microbiology and biotechnology , cycling , stimulus (psychology) , cell division , biophysics , cell , chemistry , immunology , in vitro , biochemistry , psychology , history , oceanography , archaeology , psychotherapist , geology
A mathematical model is presented for the analysis of transition between cycling and non‐cycling compartments by cells responding to a growth stimulus. the cellular age distribution as a function of time is derived from sequential [ 3 H]thymidine pulse labeling indices. Rates of entry into and exit from the cycling compartment are determined on the basis of labeling indices obtained after instantaneous and long duration [ 3 H]thymidine pulses. Analysis of an experiment involving sequential measurements over the whole lifespan of a human lymphocyte culture stimulated by phytohemagglutinin is presented as an example of the application of this method.