Open AccessSpecific Inhibition of Cell Proliferation In the Mouse Intestine By an Aqueous Extract of Rabbit Colon
Author(s) -
Sassier P.,
Bergeron M.
Publication year - 1978
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1978.tb00836.x
Subject(s) - in vivo , jejunum , small intestine , thymidine , biology , dna synthesis , kidney , homogenization (climate) , in vitro , microbiology and biotechnology , cecum , dna , biochemistry , large intestine , chemistry , medicine , endocrinology , biodiversity , ecology
Aqueous extracts from rabbit colon, kidney, testis and small intestinal mucosa were prepared by homogenization and centrifugation at 105,000 g. After precipitation with ammonium sulphate. the 0–50 fraction (F 1 ) and the supernatant (F 2 ) were collected, dialysed against a phosphate buffer and tested on mice in vivo. 1 hr after a single injection of F 1 (15 mg content) from colon, the uptake of tritiated thymidine was decreased in jejunal and colonic DNA in mice. This effect, maximal after 3 hr and totally reversible after 7 hr, was found in neither the kidney nor the testis. the F 1 fractions of non‐digestive organs (kidney, testis) were also found to exert a significant inhibition on thymidine incorporation into intestinal DNA in vivo. F 1 fractions of intestinal contents, prepared under the same conditions, exerted no significant effects on DNA synthesis in mouse intestine. Conversely, the colon F 2 fraction did not inhibit the synthesis of jejunal and colonic DNA in vivo. A slowing of cellular migration was also noticed in the jejunum and colon of mice injected with colon or small intestine F 1 , as ascertained radioautographically by determining the position of the leading edge of the labelled cells in jejunal or colonic F 1 ‐injected mice. Our results suggest that the F 1 fraction of the aqueous extract of rabbit colon contains one or more substances, which may act either on intestinal DNA synthesis or on the G 1 ‐S transition of the cellular cycle in the mouse intestine. This reversible and tissue‐specific intestinal action appears to inhibit cell proliferation and presents several of the characteristics defining a chalone, as does the action of small intestinal F 1 previously reported (Sassier & Bergeron, 1977). However, because of a relative lack of origin specificity of this effect, the physiological significance of our data remains to be ascertained.