Open AccessPluripotent (Cfu‐S) and Granulocyte‐Committed (Cfu‐C) Stem Cells In Intact and 89 Sr Marrow‐Ablated S1/S1 D Mice
Author(s) -
Adler S. S.,
Trobaugh F. E.
Publication year - 1978
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1978.tb00827.x
Subject(s) - spleen , bone marrow , microbiology and biotechnology , granulocyte , progenitor cell , femur , biology , colony forming unit , chemistry , andrology , stem cell , immunology , medicine , paleontology , genetics , bacteria
Peripheral blood values, femur cell counts, spleen weights, pluripotent (CFU‐S) and granulocyte progenitor cell (CFU‐C) concentrations and total content of spleens and femurs have been evaluated in intact (non‐marrow‐ablated) and 89 Sr marrow‐ablated S1/S1 d and +/+ mice. 89 Sr‐irradiated mice were studied 6 and 11 days after the administration of 89 Sr. In intact S1/S1 d mice the femur CFU‐S concentration, total femur CFU‐S, femur CFU‐C concentration and total femur CFU‐C were 84, 54, 105 and 68% that of +/+ mice femurs respectively; the respective values for the spleens of S1/S1 d mice were 40,46,61 and 69%. These are the first simultaneous determinations of CFU‐S and CFU‐C concentrations, and content of spleens and marrows, of S1/S1 d and +/+ mice. In 89 Sr marrow‐ablated mice, 11 days after injection of the radionuclide: (a) the total content of marrow CFU‐C and CFU‐S was about 1% of that found in the marrows of intact mice for both +/+ and S1/S1 d groups; (b) the spleens of +/+ mice increased in weight to 162% of the control, but the spleens of S1/S1 d mice did not increase in weight; and (c) the spleens of +/+ mice had a total content of CFU‐C and CFU‐S of 800% and 260% of the control, respectively, whereas the respective values for the S1/S1 d mice were 120% and 76% of the control. Thus the S1/S1 d spleen fails to compensate for marrow ablation by housing additional CFU‐S and has an impaired ability to compensate by housing additional CFU‐C.